ELK Biotechnology

Human DAPK1(Death Associated Protein Kinase 1) ELISA Kit

Product Code:
 
ELK3562
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK3562-48T48T£298.00
Quantity:
ELK3562-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
DAK1 ; DAP K1 ; DAP kinase 1 ; DAPK 1 ; DAPK ; DAPK1 ;? Death Associated Protein Kinase 1 ; Death-associated protein kinase 1 ; DKFZp781I035
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
0.062 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DAPK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DAPK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DAPK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human DAPK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P53355