ELK Biotechnology

Human RAD50(DNA Repair Protein RAD50) ELISA Kit

Product Code:
 
ELK4558
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK4558-48T48T£298.00
Quantity:
ELK4558-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
DNA repair protein RAD50; hRAD50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog; RAD50 PEN; Truncated RAD50 protein
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
0.057 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RAD50. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RAD50. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RAD50, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human RAD50 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q92878