ELK Biotechnology

Mouse HSP10(Heat Shock 10kDa Protein 1) ELISA Kit

Product Code:
 
ELK3599
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK3599-48T48T£298.00
Quantity:
ELK3599-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
HSPE1; CPN10; EPF; GROES; Chaperonin 10; Early Pregnancy Factor; 10 kDa heat shock protein, mitochondrial
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
1.57-100 ng/mL
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity:
0.65 ng/mL
Standard:
100 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse HSP10 in the samples is then determined by comparing the OD of the samples to the standard curve.