ELK Biotechnology

Rat DA(Dopamine) ELISA Kit

Product Code:
 
ELK8953
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK8953-48T48T£261.00
Quantity:
ELK8953-96T96T£335.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
4.71 pg/mL
Standard:
1000 pg/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat DA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat DA in the samples is then determined by comparing the OD of the samples to the standard curve.