ELK Biotechnology

Rat AT(Antithrombin) ELISA Kit

Product Code:
 
ELK7355
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK7355-48T48T£298.00
Quantity:
ELK7355-96T96T£390.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
AT3; ATIII; SERPINC1; Anti-Thrombin Antibodies; Serpin Peptidase Inhibitor Clade C Member 1; Coding Sequence Signal Peptide Antithrombin Part 1
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
15.63-1000 ng/mL
Sample Type:
plasma
Sensitivity:
6.8 ng/mL
Standard:
1000 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat AT in the samples is then determined by comparing the OD of the samples to the standard curve.