ELK Biotechnology

Rat PF4(Platelet Factor 4) ELISA Kit

Product Code:
 
ELK2446
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK2446-48T48T£298.00
Quantity:
ELK2446-96T96T£390.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.32-20 ng/mL
Sample Type:
serum, platelet-poor plasma and cell culture supernates
Sensitivity:
0.137 ng/mL
Standard:
20 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P06765