ELK Biotechnology

Simian α2M(Alpha-2-Macroglobulin) ELISA Kit

Product Code:
 
ELK8292
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Simian
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK8292-48T48T£359.00
Quantity:
ELK8292-96T96T£477.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
7.82-500 ng/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
3.5 ng/mL
Standard:
500 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian α2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian α2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian α2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Simian α2M in the samples is then determined by comparing the OD of the samples to the standard curve.