NSJ Bioreagents

PCM1 Antibody / Pericentriolar material 1 protein

Product Code:
 
NSJ-F54931
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Pericentriolar material 1 protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human Ramos cell lysate with Pericentriolar material 1 protein antibody. Predicted molecular weight ~229 kDa.
2 / 4
IHC testing of FFPE human breast carcinoma tissue with Pericentriolar material 1 protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human A549 cells with Pericentriolar material 1 protein antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human Ramos cells with Pericentriolar material 1 protein antibody; Blue=isotype control, Green= Pericentriolar material 1 protein antibody.
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Western blot testing of human Ramos cell lysate with Pericentriolar material 1 protein antibody. Predicted molecular weight ~229 kDa.
IHC testing of FFPE human breast carcinoma tissue with Pericentriolar material 1 protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human A549 cells with Pericentriolar material 1 protein antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human Ramos cells with Pericentriolar material 1 protein antibody; Blue=isotype control, Green= Pericentriolar material 1 protein antibody.

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CodeSizePrice
NSJ-F54931-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54931-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 632-661 from the human protein was used as the immunogen for the Pericentriolar material 1 protein antibody.
Limitation:
This Pericentriolar material 1 protein antibody is available for research use only.
Localization:
Cytoplasmic, centrosome
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q15154