NSJ Bioreagents

p19-INK4d Antibody / CDKN2D

Product Code:
 
NSJ-F55034
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CDKN2D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human Jurkat cell lysate with CDKN2D antibody. Predicted molecular weight ~18 kDa.
2 / 4
Western blot testing of mouse testis tissue lysate with CDKN2D antibody. Predicted molecular weight ~18 kDa.
3 / 4
IHC testing of FFPE human testis tissue with CDKN2D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HeLa cells with CDKN2D antibody; Blue=isotype control, Green= CDKN2D antibody.
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Western blot testing of human Jurkat cell lysate with CDKN2D antibody. Predicted molecular weight ~18 kDa.
Western blot testing of mouse testis tissue lysate with CDKN2D antibody. Predicted molecular weight ~18 kDa.
IHC testing of FFPE human testis tissue with CDKN2D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with CDKN2D antibody; Blue=isotype control, Green= CDKN2D antibody.

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CodeSizePrice
NSJ-F55034-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55034-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Description:
p19 / Cyclin-dependent kinase 4 inhibitor D is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 15-43 from the human protein was used as the immunogen for the CDKN2D antibody.
Limitation:
This CDKN2D antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P55273