NSJ Bioreagents

Negative Control Antibody for Mouse Monoclonals

Product Code:
 
NSJ-V8785
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
IGG1/453
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Aliquot the Negative control antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human heart tissue with Negative control antibody (clone IGG1/453). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free Neg

IHC staining of FFPE human heart tissue with Negative control antibody (clone IGG1/453). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free Neg

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CodeSizePrice
NSJ-V8785-20UG20ug£264.00
Quantity:
NSJ-V8785-100UG100ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
ELISA; Flow cytometry; Immunofluorescence; Immunoprecipitation; Western blot; Immunohistochemistry: use at the same concentration as the primary antibody being controlled for
Application Note:
Optimal dilution of the Negative control antibody should be determined by the researcher.
Description:
To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human IG1R protein was used as the immunogen for the Negative control antibody.
Limitation:
This Negative control antibody is available for research use only.
Purity:
Protein A affinity
Uniprot #:
Not Applicable