CD152 [CTLA-4] (human) (rec.) (His)

Cytotoxic T-lymphocyte-associated Antigen 4; Cytotoxic T-lymphocyte Protein 4

Binds to human CD80.

1mg/ml after reconstitution.

32160000

<0.01EU/µg purified protein (LAL test).

solid

Lyophilized. Contains PBS.

After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Recombinant Protein. Human CD152 [CTLA-4] (aa 36-161) is fused at the C-terminus to a His-tag. Source: W2. Lyophilized. Contains PBS. CD152 (Cytotoxic T-lymphocyte-associated protein 4; CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. While CD28 promotes T-cell activation and proliferation, CTLA-4 is reported to dampen T-cell responses through a variety of mechanisms. Prior to activation, conventional T-cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T-cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane. Recently, roles of CTLA-4 have been demonstrated in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T-cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research.

~20kDa (SDS-PAGE)

P16410

Plastic Vial

CD152 (Cytotoxic T-lymphocyte-associated protein 4; CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. While CD28 promotes T-cell activation and proliferation, CTLA-4 is reported to dampen T-cell responses through a variety of mechanisms. Prior to activation, conventional T-cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T-cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane. Recently, roles of CTLA-4 have been demonstrated in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T-cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research.

>95% (SDS-PAGE)

Human CD152 [CTLA-4] (aa 36-161) is fused at the C-terminus to a His-tag.

HEK 293 cells

His

Non-hazardous

Other Proteins

12352202

Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.