ELK Biotechnology

Chicken 8-iso-PGF2α(8-isoprostane) ELISA Kit

Product Code:
 
ELK9298
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Chicken (Gallus)
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9298-48T48T£298.00
Quantity:
ELK9298-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
8-isoprostane; 8-iso-PGF2α
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
15.625-1000 pg/mL
Research Area:
Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
9.375 pg/mL
Standard:
1000 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-isoprostane(8-iso-PGF2α) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-isoprostane(8-iso-PGF2α). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-isoprostane(8-iso-PGF2α) in the samples is then determined by comparing the OD of the samples to the standard curve.