Human GALP(Galanin-like peptide) ELISA Kit

Galanin like peptide; Galanin like peptide precursor protein; Alarin splice variant; Alarin; GALP precursor protein

3.5h

Sandwich

78.13-5000 pg/mL

Signal transduction; Neuroscience; Cardiovascular; Developmental Biology

Serum, plasma, tissue homogenates and other biological fluids.

29 pg/mL

5000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galanin-like peptide(GALP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Galanin-like peptide(GALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galanin-like peptide(GALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galanin-like peptide(GALP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Q9UBC7