ELK Biotechnology

Sheep Bcl2(B-Cell Leukemia/Lymphoma 2) ELISA Kit

Product Code:
 
ELK9361
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Sheep
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9361-48T48T£359.00
Quantity:
ELK9361-96T96T£477.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
B-Cell CLL/Lymphoma 2
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
1.57-100 ng/mL
Research Area:
Signal transduction;Apoptosis;
Sample type:
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:
0.55 ng/mL
Standard:
100 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2(Bcl2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2(Bcl2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Leukemia/Lymphoma 2(Bcl2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Leukemia/Lymphoma 2(Bcl2) in the samples is then determined by comparing the OD of the samples to the standard curve.