ELK Biotechnology

Cattle T4(Thyroxine) ELISA Kit

Product Code:
 
ELK9521
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Bovine (Cattle)
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9521-48T48T£261.00
Quantity:
ELK9521-96T96T£335.00
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
4.69-300 ng/mL
Research Area:
Endocrinology;Hormone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
1.42 ng/mL
Standard:
300 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroxine(Cattle T4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroxine(Cattle T4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroxine(Cattle T4) in the samples is then determined by comparing the OD of the samples to the standard curve.