ELK Biotechnology

Human N-MID-OT(N-MIDOsteocalcin) ELISA Kit

Product Code:
 
ELK9587
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9587-48T48T£298.00
Quantity:
ELK9587-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
N-MID-OT
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.63-40 ng/mL
Research Area:
Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.38 ng/mL
Standard:
40 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-MIDOsteocalcin(N-MID-OT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-MIDOsteocalcin(N-MID-OT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-MIDOsteocalcin(N-MID-OT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-MIDOsteocalcin(N-MID-OT) in the samples is then determined by comparing the OD of the samples to the standard curve.