ELK Biotechnology

Simian NTXI(CrossLinked N-telopeptide of Type I Collagen) ELISA Kit

Product Code:
 
ELK9626
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Simian
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9626-48T48T£298.00
Quantity:
ELK9626-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
NTX-I
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Signal Transduction?Metabolism
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.09 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CrossLinked N-telopeptide of Type I Collagen(NTXI). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CrossLinked N-telopeptide of Type I Collagen(NTXI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CrossLinked N-telopeptide of Type I Collagen(NTXI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CrossLinked N-telopeptide of Type I Collagen(NTXI) in the samples is then determined by comparing the OD of the samples to the standard curve.