ELK Biotechnology

Rabbit GAPDH(Glyceraldehyde-3-Phosphate Dehydrogenase) ELISA Kit

Product Code:
 
ELK9679
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rabbit
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9679-48T48T£298.00
Quantity:
ELK9679-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
1.57-100 ng/mL
Research Area:
Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Hepatology;Nutrition metabolism;
Sample type:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.56 ng/mL
Standard:
100 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P46406