ELK Biotechnology

Rabbit TAT(Thrombin/Antithrombin Complex) ELISA Kit

Product Code:
 
ELK9696
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rabbit
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9696-48T48T£298.00
Quantity:
ELK9696-96T96T£390.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
Thrombin/Antithrombin Complex
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.32-20 ng/mL
Research Area:
Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology;
Sample type:
Plasma
Sensitivity:
0.117 ng/mL
Standard:
20 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombin/Antithrombin Complex(TAT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thrombin/Antithrombin Complex(TAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombin/Antithrombin Complex(TAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombin/Antithrombin Complex(TAT) in the samples is then determined by comparing the OD of the samples to the standard curve.