ELK Biotechnology

Human SCG10(Superior cervical ganglia neural specific 10) ELISA Kit

Product Code:
 
ELK9757
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9757-48T48T£261.00
Quantity:
ELK9757-96T96T£335.00
Quantity:
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Further Information

Alternative Names:
STMN2; SCG10; SCGN10; Stathmin-2; Superior cervical ganglion-10 protein; Protein SCG10
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Neuroscience
Sample type:
serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.058 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superior cervical ganglia neural specific 10(SCG10). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Superior cervical ganglia neural specific 10(SCG10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superior cervical ganglia neural specific 10(SCG10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superior cervical ganglia neural specific 10(SCG10) in the samples is then determined by comparing the OD of the samples to the standard curve.