ELK Biotechnology

Mouse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Product Code:
 
ELK9776
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9776-48T48T£298.00
Quantity:
ELK9776-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
TBARS; Thiobarbituric Acid Reactive Substance
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
6.25-400 nmol/ml
Research Area:
Metabolic pathway;Hepatology;Hormone metabolism;
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity:
3.55 nmol/ml
Standard:
400nmol/ml
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thiobarbituric Acid Reactive Substance(TBARS) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thiobarbituric Acid Reactive Substance(TBARS). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thiobarbituric Acid Reactive Substance(TBARS) in the samples is then determined by comparing the OD of the samples to the standard curve.