ELK Biotechnology

Rat Phospho-Akt (S473) ELISA Kit

Product Code:
 
ELK9777
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

No additional charges, what you see is what you pay! *

CodeSizePrice
ELK9777-48T48T£298.00
Quantity:
ELK9777-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Videos
  • Further Information
  • Show All

Video

Further Information

Alternative Names:
p-AKT
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
15.63-1000 pg/mL
Research Area:
Enzyme & Kinase;
Sample type:
tissue homogenates, cell lysates and other biological fluids
Sensitivity:
6.7 pg/mL
Standard:
1000 pg/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospho-Akt (S473). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phospho-Akt (S473). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospho-Akt (S473), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospho-Akt (S473) in the samples is then determined by comparing the OD of the samples to the standard curve.