TNF-beta Antibody (biotin)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Tumor Necrosis Factor-beta, TNFSF1B, Lymphotoxin-alpha (LT-alpha)
Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human TNF-beta as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human TNF-beta.
Western Blot
To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells. Recombinant Human TNF-beta is a secreted 172 amino acid protein (18.6 kDa) which forms an active non-disulfide-linked homotrimer structure in solution.
batch dependent
Biotin
Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-beta. Anti-Human TNF-beta specific antibody was purified by affinity chromatography and then biotinylated.
4049
lymphotoxin alpha
LTA
Homo sapiens
Lyophilized
P01374
P01374.2
TNF-beta specific antibody was purified by affinity chromatography and then biotinylated
Immunology,Apoptosis,Obesity,Neuroscience,Cell Cycle,Stem Cell,Cancer
P01374
Centrifuge vial prior to opening.