ProSci

Amphiregulin Antibody (biotin)

Product Code:
 
PSI-38-369
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect Human Amphiregulin by sandwich ELISA (using 100ul/well), a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Amphiregulin as a capture antibody, allows the detection of at least 2000-4000 pg/ml ofRecombinant Human Amphiregulin.
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To detect Human Amphiregulin by sandwich ELISA (using 100ul/well), a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Amphiregulin as a capture antibody, allows the detection of at least 2000-4000 pg/ml ofRecombinant Human Amphiregulin.

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CodeSizePrice
PSI-38-369-0.025mg0.025mg£505.00
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Further Information

Additional Names:
AR, AREG, Colorectum cell-derived growth factor (CRDGF)
Application Note:
Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human Amphiregulin as a capture antibody, allows the detection of at least 2000-4000 pg/ml ofRecombinant Human Amphiregulin.
Western Blot
To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-alpha receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin?s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant Human Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
374
NCBI Official Name:
amphiregulin
NCBI Official Symbol:
AREG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P15514
Protein GI Number:
P15514.2
Purification:
Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Cancer,Other,Immunology,Neuroscience
Swissprot #:
P15514
User NOte:
Centrifuge vial prior to opening.