RevMab Biosciences

5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair

Product Code:
 
REV-31-1354-MK
Product Group:
 
ELISA Kits
Supplier:
 
RevMab Biosciences
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Shipping:
 
BLUE ICE
Storage:
 
Long term storage:-20°C.
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Detection of 5-Carboxylcytosine (5-caC), in linear denatured DNA (426 bp). Sandwich ELISA using anti-Carboxylcytosine (5-caC) (clone RM462) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.
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Detection of 5-Carboxylcytosine (5-caC), in linear denatured DNA (426 bp). Sandwich ELISA using anti-Carboxylcytosine (5-caC) (clone RM462) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.

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REV-31-1354-MK-KI011 Set£760.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
5-caC
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Avoid freeze/thaw cycles.
Kit Contains:
1) Capture Antibody: Anti-5-Carboxylcytosine (5-caC) Rabbit Monoclonal Antibody, Clone RM462, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Long Description:
Matched Pair Detection Set. This antibody pair detects 5-Carboxylcytosine (5-caC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Package Type:
Vial
Product Description:
5-Carboxylcytosine (5-caC) is a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
Specificity:
This antibody pair detects 5-Carboxylcytosine (5-caC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Transportation:
Non-hazardous
UNSPSC Number:
41116126
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

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