APOPCYTO Caspase-8 Colorimetric Assay Kit

Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates.

Human: 22861 Mouse: 12370

Cell Lysis Buffer, 2X Reaction Buffer, 1M DTT, Substrate IETD-pNA (10mM), Inhibitor IETD-FMK (1mM), 100mMpNA (p-nitroanilide)

see label

Caspase-8