Ab-Match ASSEMBLY Human PAP1 (REG3alpha) kit

REG3A, HIP, INGAP, PAP, PAP-H, PAP1, PBCGF, REG-III, REG3

PAP1 (pancreatitis associated protein 1) is membrane protein which is overexpressed in pancreas during acute pancreatitis (Ref.1). Amylase, lipase, and tripsin are often used as serum markers for pancreatitis, but these markers only show a transient increase in serum. The blood concentration of PAP1, in contrast, correlates well with the severity of pancreatitis. Therefore, PAP 1 can be used as prognostic marker for pancreatitis (Ref.2). Elevated serum PAP1 has been reported not only in pancreatic cancer, but also in colon and liver cancer (Ref.3,4). MBL?s PAP1 ELISA kit can detect antigen in culture supernatant for the colon cancer derived cell line OUMS-23.

The anti-PAP1 capture antibody is coated on a 96 microwell plate. Standards or samples are added to the microwells, allowing PAP1 to bind to the capture antibody in proportion to the concentration of PAP1 in the sample (primary reaction). After wash unbound PAP1, biotin conjugated anti-PAP1 detection antibody is added and attaches to the PAP1 bound to coated antibody (secondary reaction). After washing away all unbound detection antibody, peroxidase conjugated streptavidin (SA-HRP) is added and attaches to the biotin label on the bound detection antibody (biotin-avidin reaction). Another wash removes all unbound SA-HRP, before the substrate is added the peroxidase reaction. After adding the stop solution to the microwell plate, the relative amount of PAP1 bound in each well can be determined from the yellow color using a microplate reader. The concentration of PAP1 in each sample can be obtained by comparing the OD of the sample to the OD of the standards on the plate.

Human: 5068 Mouse: 18489

PAP1 Standard, Anti-PAP1 capture antibody, Anti-PAP1 detection antibody, Streptavidin conj. Peroxidase

1 year

PAP1