CircuLex CML/Nε-Carboxymethyl)lysine ELISA Kit
Product Code:
MBL-CY-8066
MBL-CY-8066
Regulatory Status:
RUO
RUO
Application:
Enzyme-Linked Immunosorbent Assay (ELISA)
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
4°C
Storage:
4°C
4°C
No additional charges, what you see is what you pay! *
| Code | Size | Price |
|---|
| MBL-CY-8066 | 96 Assays | £602.00 |
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Typical lead time: 10-14 working days.
Contact us for more accurate information.
- Further Information
- Documents
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Further Information
Background:
Reducing sugars react with protein amino groups to form a diverse group of protein-bound moieties with fluorescent and cross-linking properties. These compounds, called advanced glycosylation end products (AGEs), have been implicated in the structural and functional alterations of proteins that occur during aging and long-term diabetes.
Although several AGE structures have been reported (1, 2), it was demonstrated that Nε-(carboxymethyl) lysine (CML) is a major antigenic AGE structure. CML concentration is also increased in patients who have diabetes with complications, including nephropathy (3?5), retinopathy (6), and atherosclerosis (7?9). CML is also recognized by receptor for AGE (RAGE), and CML-RAGE interaction activates cell signaling pathways such as NF-B and enhances the expression of vascular cell adhesion molecule-1 in human umbilical vein endothelial cells (10).
Description:
The CycLex Research Product CircuLex CML/Nε-(carboxymethyl) lysine ELISA kit is used for the
quantitative measurement of CML-adducts in mammalian serum, plasma, tissue extract and other
biological media except rodent specimen.
Gene IDs:
Human: 613 Mouse: 110279
Kit Components:
CML-BSA coated Microplate, Wash Buffer, Sample Dilution Buffer, Standard Dilution Buffer, CML-HSA Standard, Primary Antibody, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution
Measurement Range:
Results exceeding CMLtoadduct level of 20 ug/mL CMLtoHSA should be repeated with diluted samples. Dilution factors need to be taken into consideration in calculating the CMLtoHSA concentration.
Sensitivity:
better than 0.063 ug/mL of sample.
Documents
References
(1) Ikeda K, Higashi T, Sano H, Jinnouchi Y, Yoshida M, Araki T, Ueda S, Horiuchi S: Biochemistry 35: 8075 ?8083,1996
(2) Reddy S, Bichler J, Wells-Knecht KJ, Thorpe SR, Baynes JW: Biochemistry 34: 10872 ?10878,1995
(3) Makino H, Shikata K, Hironaka K, Kushiro M, Yamasaki Y, Sugimoto H, Ota Z, Araki N, Horiuchi S: Kidney Int 48: 517 ?526,1995
(4) Suzuki D, Yagame M, Jinde K, Naka R, Yano N, Endoh M, Kaneshige H, Nomoto Y, Sakai H: J Diabetes Complications 10: 314 ?319,1996
(5) Imai N, Nishi S, Suzuki Y, Karasawa R, Ueno M, Shimada H, Kawashima S, Nakamaru T, Miyakawa Y, Araki N, Horiuchi S, Gejyo F, Arakawa M: Nephron 76: 153 ?160,1997
(6) Murata T, Nagai R, Ishibashi T, Inomuta H, Ikeda K, Horiuchi S: Diabetologia 40: 764 ?769,1997
(7) Kume S, Takeya M, Mori T, Araki N, Suzuki H, Horiuchi S, Kodama T, Miyauchi Y, Takahashi K: Am J Pathol. 147: 654 ?667,1995
(8) Sakata N, Imanaga Y, Meng J, Tachikawa Y, Takebayashi S, Nagai R, Horiuchi S, Itabe H, Takano T: Atherosclerosis 141: 61 ?75,1998
(9) Sakata N, Imanaga Y, Meng J, Tachikawa Y, Takebayashi S, Nagai R, Horiuchi S: Atherosclerosis 142: 67 ?77,1999
(10) Kislinger T, Fu C, Huber B, Qu W, Taguichi A, Du Yan S, Hofmann M, Yan SF, Pischetsrieder M, Stern D, Schmidt AM: J Biol Chem 274: 31740 ?31749,1999