Rat INS(Insulin) ELISA Kit

INS

3.5h

This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed

0.31-20 ng/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

0.19 ng/mL

Rat

IDDM2;ILPR;IRDN;MODY10;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the OD of the samples to the standard curve.

cell culture supernatant and tissue homogenate

Sandwich

P01322,P01323

This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in cell culture supernatant and tissue homogenate.