Elabscience

GAPDH Polyclonal Antibody

Product Code:
 
E-AB-40516
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
  • Zebrafish
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot of Zebrafish whole lysates with anti-GAPDH at dilution of 1:2000.
2 / 2
Immunofluorescence analysis of 293 cell, using anti-GAPDH Polyclonal Antibody at 1:400 dilution.
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Western blot of Zebrafish whole lysates with anti-GAPDH at dilution of 1:2000.
Immunofluorescence analysis of 293 cell, using anti-GAPDH Polyclonal Antibody at 1:400 dilution.

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E-AB-40516-20uL20uL£110.00
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E-AB-40516-50uL50uL£143.00
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E-AB-40516-100uL100uL£177.00
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E-AB-40516-200uL200uL£210.00
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Further Information

Abbreviation:
GAPDH
Background:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Calculated MW:
40.2kDa
Concentration:
2mg/mL
Conjugation:
Unconjugated
Dilution:
WB: 1:1000-2000, IF: 1:100-400
Immunogen:
Recombinant human GAPDH protein expressed by E.coli
ObservedMW:
36kDa
Purification method:
Antigen Affinity Purification
Target Synonym:
aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component
UNIProt ID:
Q5XJ10

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S1050464822005952