Elabscience

Sucrose Colorimetric Assay Kit

Product Code:
 
E-BC-K161-S
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Quantification
Shipping:
 
Ice packs
Storage:
 
2-8°C
1 / 2
2 / 2
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CodeSizePrice
E-BC-K161-S-50Assays50Assays£197.00
Quantity:
E-BC-K161-S-100Assays100Assays£251.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Assay Time:
40 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.32-70 umol/mL
Detection Type:
Colorimetric method
Inter cv:
8.5
Intra cv:
3.4
Result Type:
Quantitative
Sensitivity:
0.32 umol/mL
Test Principle:
Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Tested Sample Types:
Plant tissue

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