Elabscience

SARS-CoV-2 Nucleocapsid Protein ELISA Kit

Product Code:
 
E-EL-E604
Product Group:
 
ELISA Kits
Supplier:
 
Elabscience
Host Type:
 
Virus
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Ice packs
Storage:
 
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month store the items separately according to the storage table in the manual once the kit is received.
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Standard curve
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Standard curve

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CodeSizePrice
E-EL-E604-96T96T£371.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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This product comes from: China.
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  • Further Information
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Further Information

Abbreviation:
Nprotein
Assay Time:
3.5h
Cross reactivity:
This kit recognizes SARS-CoV-2 N protein in samples.No significant cross-reactivity or interference between SARS-CoV-2 N protein and analogues was observed
Detection Range:
0.39-25 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.12 ng/mL
Target Species:
SARS-CoV-2
Target Synonym:
N protein;Protein N;NP;NC;Nucleoprotein;2019-nCoV N protein;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 N protein. Samples (or Standards) and biotinylated detection antibody specific for SARS-CoV-2 N protein are added to the micro ELISA plate wells. SARS-CoV-2 N protein would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 N protein, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ? 2 nm. The OD value is proportional to the concentration of SARSCoV-2 N protein. You can calculate the concentration of SARS-CoV-2 N protein in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P0DTC9
Usage:
This ELISA kit applies to the in vitro Quantitative determination of N protein in serum or plasma.

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0956566321005728