SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit

SARS-CoV-2 IgM

1.5h

This kit recognizes Human SARS-CoV-2 IgM in samples.No significant cross-reactivity or interference between Human SARS-CoV-2 IgM and analogues was observed

Colormetric

Intra-CV: CV%<8%; Inter-CV: CV%<10%

80%-120%

Human

COVID 19 IgM;N protein IgM;

This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein (E-ELC-001) antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ? 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgM exists in the tested samples or not.

serum, plasma

Indirect

This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma.