Elabscience

Bcl-2 Polyclonal Antibody

Product Code:
 
E-AB-60012
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of HL-60 cells using Bcl-2 Polyclonal Antibody at dilution of 1:500.
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Western blot analysis of extracts of various cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000.
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Immunohistochemistry of paraffin-embedded Human lung cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of HL-60 cells using Bcl-2 Polyclonal Antibody at dilution of 1:500.
Western blot analysis of extracts of various cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human lung cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-60012-60uL60uL£210.00
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E-AB-60012-120uL120uL£291.00
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E-AB-60012-200uL200uL£431.00
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  • Further Information
  • Documents
  • References
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Further Information

Abbreviation:
Bcl-2
Background:
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
22kDa/26kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:100
Immunogen:
A synthetic peptide of human Bcl-2
ObservedMW:
24kDa/26kDa
Purification method:
Affinity purification
Target Synonym:
Bcl-2;PPP1R50;BCL2
UNIProt ID:
P10415

Documents

References

  • https://www.mdpi.com/1806946
  • https://www.mdpi.com/1281194
  • https://link.springer.com/article/10.1007/s11356-023-26018-0
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/jre.12973
  • https://www.sciencedirect.com/science/article/pii/S0300483X18303937
  • https://www.mdpi.com/2080828
  • https://www.sciencedirect.com/science/article/pii/S0006291X19321874
  • https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-019-2768-1
  • https://link.springer.com/article/10.1007/s12011-018-1587-4