Recombinant Mouse Interleukin-22/IL-22 Protein (Active)

Interleukin-22;IL-22

Q9JJY9

Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is 281. 45 pg/mL.

Interleukin-22 (IL-22) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acid signal peptide that is cleaved to generate a 147 amino acid mature protein that shares approximately 79% and 22% sequence identity with human IL22 and IL10, respectively. IL22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and up-regulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family.

16.7 kDa

< 1.0 EU per ug of the protein as determined by the LAL method.

E.coli

Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

None

15 kDa

> 95 % as determined by reducing SDS-PAGE.

Leu34-Val179

Interleukin-22;IL-22;IL-10-Related T-Cell-Derived-Inducible Factor;IL-TIF;IL-TIF Alpha;Interleukin-22a;IL-22a;Il22;Il22a;Iltif;Iltifa

Q9JJY9