Recombinant Mouse Interleukin-13/IL-13 Protein (aa 26-131,His Tag)(Active)

Interleukin-13;IL-13

P20109

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2-13 ng/ml.

Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.

12.7 kDa

< 1.0 EU per ug of the protein as determined by the LAL method.

HEK293 Cells

Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

C-6His

15-30 kDa

> 95 % as determined by reducing SDS-PAGE.

Ser26-Phe131

Interleukin-13;IL-13;T-Cell Activation Protein P600;Il13;Il-13

P20109