Elabscience

Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)(Active)

Product Code:
 
PKSH030445
Product Group:
 
Recombinant Proteins
Supplier:
 
Elabscience
Host Type:
 
Human
Regulatory Status:
 
RUO
Applications:
  • Cell Culture
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
 
Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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CodeSizePrice
PKSH030445-5ug5ug£179.00
Quantity:
PKSH030445-100ug100ug£960.00
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This product comes from: China.
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Further Information

Abbreviation:
IL-23
Accession:
Q9NPF7&NP_002178.2
Activity:
1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Immobilized human IL23A-His+IL12B-His at 10 ug/ml (100 ul/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 ug/ml. 3. Immobilized human IL23A-His+IL12B-His at 10 ug/ml (100 ul/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 ug/ml. 4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL.
Background:
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
Calculated MW:
56.3 (20.1 + 36.2) kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
22&45 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Pro189&Met 1-Ser 28
Target Synonym:
SGRF;IL-23p19;CLMF p40;IL-12 subunit p40;NKSF2;IL-23;IL-23A;IL23P19;P19

Documents