Elabscience

PKM1-specific Polyclonal Antibody

Product Code:
 
E-AB-92126
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines using PKM1-specific Polyclonal Antibody at 1:1000 dilution.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using PKM1-specific Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
3 / 4
Immunofluorescence analysis of HeLa cells using PKM1-specific Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of NIH/3T3 cells using PKM1-specific Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines using PKM1-specific Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using PKM1-specific Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using PKM1-specific Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PKM1-specific Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
E-AB-92126-60uL60uL£210.00
Quantity:
E-AB-92126-120uL120uL£291.00
Quantity:
E-AB-92126-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Abbreviation:
PKM1-specific
Background:
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
56kDa/57kDa/58kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human PKM1-specific
ObservedMW:
58kDa
Purification method:
Affinity purification
Target Synonym:
PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1;
UNIProt ID:
P14618

Documents