Elabscience

Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit

Product Code:
 
E-BC-K071-S
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
2-8°C
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E-BC-K071-S-50Assays50Assays£197.00
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Further Information

Assay Time:
50 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.03-50 mg/L
Detection Type:
Colorimetric method
Inter cv:
4.7
Intra cv:
3.4
Result Type:
Quantitative
Sensitivity:
0.03 mg/L
Target Synonym:
TIBC
Test Principle:
The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC).
Tested Sample Types:
Serum

Documents

References

  • https://pubs.rsc.org/en/content/articlelanding/2023/fo/d2fo02226e
  • https://www.sciencedirect.com/science/article/pii/S0946672X21000845