HS Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

TNF-α

3.5h

This kit recognizes Rat TNF-α in samples. No significant cross-reactivity or interference between Rat TNF-α and analogues was observed.

1.56-100 pg/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

0.86 pg/mL

Rat

DIF; TNF-alpha; TNFA; TNFSF2

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Rat TNF-α. You can calculate the concentration of Rat TNF-α in the samples by comparing the OD of the samples to the standard curve.

serum, plasma and other biological fluids

Sandwich

P16599

This ELISA kit applies to the in vitro quantitative determination of Rat TNF-α concentrations in serum, plasma and other biological fluids.