NSJ Bioreagents

AMACR / p504S Antibody (Prostate Cancer Marker)

Product Code:
 
NSJ-V9306SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rAMACR/6369
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant AMACR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human prostate tissue with recombinant AMACR antibody (clone rAMACR/6369). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human kidney lysate using recombinant AMACR antibody (clone rAMACR/6369). Predicted molecular weight ~43 kDa.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant AMACR antibody (clone rAMACR/6369) as confirmation of integrity and purity.

IHC staining of FFPE human prostate tissue with recombinant AMACR antibody (clone rAMACR/6369). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human kidney lysate using recombinant AMACR antibody (clone rAMACR/6369). Predicted molecular weight ~43 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant AMACR antibody (clone rAMACR/6369) as confirmation of integrity and purity.

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NSJ-V9306SAF-100UG100ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant AMACR antibody should be determined by the researcher.
Description:
This antibody recognizes a protein of 42kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human AMACR protein was used as the immunogen for the recombinant AMACR antibody.
Limitation:
This recombinant AMACR antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A/G affinity
Uniprot #:
Q9UHK6

Documents