NSJ Bioreagents

YB1 Antibody / Y-box-binding protein 1

Product Code:
 
NSJ-V7984SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
YBX1/2430
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the YB-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC staining of FFPE human prostate carcinoma with YB-1 antibody (clone YBX1/2430). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using YB-1 antibody (clone YBX1/2430). These results demonstrate the foremost specificity of the YBX1/2430 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
3 / 3
SDS-PAGE analysis of purified, BSA-free YB-1 antibody (clone YBX1/2430) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human prostate carcinoma with YB-1 antibody (clone YBX1/2430). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using YB-1 antibody (clone YBX1/2430). These results demonstrate the foremost specificity of the YBX1/2430 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free YB-1 antibody (clone YBX1/2430) as confirmation of integrity and purity.

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NSJ-V7984SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Y-box binding protein-1 (YBX1) is the prototypic member of the cold shock protein family that fulfills numerous cellular functions. In the nucleus, YBX1 protein orchestrates transcription of proliferation-related genes, whereas in the cytoplasm it associates with mRNA and directs translation. In human tumor entities, such as breast, lung and prostate cancer, cellular YBX1 expression indicates poor clinical outcome, suggesting that YBX1 is an attractive marker to predict patients' prognosis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human full-length YBX1 protein was used as the immunogen for this YB-1 antibody.
Limitation:
This YB-1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P67809

Documents