NSJ Bioreagents

TNF alpha Antibody

Product Code:
 
NSJ-V7163SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgM kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TMDa1-1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the TNF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Immunofluorescent staining of PFA-fixed human HepG2 cells with TNF-alpha antibody (green, clone TMDa1-1) and Reddot nuclear stain (red).~
2 / 2
IHC testing of FFPE human histiocytoma and TNF alpha antibody (clone TMDa1-1). Staining of formalin
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Immunofluorescent staining of PFA-fixed human HepG2 cells with TNF-alpha antibody (green, clone TMDa1-1) and Reddot nuclear stain (red).~
IHC testing of FFPE human histiocytoma and TNF alpha antibody (clone TMDa1-1). Staining of formalin

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NSJ-V7163SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Titering of the TNF alpha antibody may be required for optimal performance.
Description:
Tumor Necrosis Factor Alpha (TNF alpha) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length human recombinant protein was used as the immunogen for the TNF alpha antibody.
Limitation:
This TNF alpha antibody is available for research use only.
Localization:
Cytoplasmic and extracellular (secreted)
Purity:
PEG precipitation
Uniprot #:
P01375

Documents