NSJ Bioreagents

Rabbit IgG Fab Antibody

Product Code:
 
NSJ-R20172
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Caprine (Goat)
Antibody Isotype:
 
Goat IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RMG01
Regulatory Status:
 
RUO
Target Species:
 
Rabbit
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Store the recombinant Rabbit IgG Fab antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
1 / 3
Western blot of nonreduced(-) and reduced(+) rabbit IgG (20ng/lane), using 0.2ug/ml of recombinant Rabbit IgG Fab antibody. This antibody reacts to nonreduced rabbit IgG (~150 kDa).
2 / 3
ELISA of IgGs from different species shows the recombinant Rabbit IgG Fab antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human/mouse/rat IgG.
3 / 3
ELISA Titration: the plate was coated with different amounts of rabbit IgG. A serial dilution of the recombinant Rabbit IgG Fab antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
❮❯

Western blot of nonreduced(-) and reduced(+) rabbit IgG (20ng/lane), using 0.2ug/ml of recombinant Rabbit IgG Fab antibody. This antibody reacts to nonreduced rabbit IgG (~150 kDa).
ELISA of IgGs from different species shows the recombinant Rabbit IgG Fab antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human/mouse/rat IgG.
ELISA Titration: the plate was coated with different amounts of rabbit IgG. A serial dilution of the recombinant Rabbit IgG Fab antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.

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CodeSizePrice
NSJ-R20172-100UG100ug£513.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
ELISA: 0.01ug/ml - 0.5ug/ml,Western Blot (non-reduced): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Rabbit IgG Fab antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Rabbit IgG Fab antibody reacts to the Fab region of Rabbit IgG. No cross reactivity with human/mouse/rat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Rabbit IgG was used as the immunogen for this recombinant Rabbit IgG Fab antibody.
Limitation:
This recombinant Rabbit IgG Fab antibody is available for research use only.
Purity:
Protein G purified from animal origin-free supernatant

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