MUC1 Antibody / Mucin-1

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Titration of the recombinant Mucin-1 may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

4582

Human milk fat globule membranes were used as the immunogen for this recombinant Mucin-1 antibody.

This recombinant Mucin-1 antibody is available for research use only.

Cytoplasmic and cell surface

Protein A affinity chromatography

P15941