NSJ Bioreagents

NKX2.1 Antibody / TTF-1

Product Code:
 
NSJ-V7084SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
HBNK2-1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human thyroid tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.~
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IHC testing of FFPE human lun
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IHC testing of FFPE human thyroid tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.~
IHC testing of FFPE human lun

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NSJ-V7084SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the TTF-1 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant protein was used as the immunogen for the TTF-1 antibody.
Limitation:
This TTF-1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P43699

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