NSJ Bioreagents

HER2 Antibody / ErbB2

Product Code:
 
NSJ-V3838SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ERBB2/2453
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human breast carcinoma with HER2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
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Analysis of HuProt(TM) microarray containing more than 19,000 ful
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IHC testing of FFPE human breast carcinoma with HER2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
Analysis of HuProt(TM) microarray containing more than 19,000 ful

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NSJ-V3838SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.
Description:
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 311-462 from the human protein was used as the immunogen for this HER2 antibody.
Limitation:
This HER2 antibody is available for research use only.
Localization:
Cell membrane (This mAb binds to the extracellular/cell surface region of the protein)
Purity:
Protein G affinity chromatography
Uniprot #:
P04626

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