NSJ Bioreagents

EGF Receptor / EGFR Antibody

Product Code:
 
NSJ-V7040SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ERB59-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green, clone ERB59-1) with DAPI nuclear stain (blue).~
2 / 2
FACS testing of A431 cells with isotype control (gray), without primary antibody (black) and EGFR an
❮❯

Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green, clone ERB59-1) with DAPI nuclear stain (blue).~
FACS testing of A431 cells with isotype control (gray), without primary antibody (black) and EGFR an

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V7040SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.
Description:
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
1956
Immunogen:
Recombinant human protein was used as the immunogen for this EGFR antibody.
Limitation:
This EGFR antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P00533

Documents