Macrophage / Histiocytoma Marker Antibody

Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Optimal dilution of the Macrophage / Histiocytoma Marker antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this mAb and a standard CD68 mAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This mAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This mAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A membrane preparation from human hepatocytes was used as the immunogen for the Macrophage / Histiocytoma Marker antibody.

This Macrophage / Histiocytoma Marker antibody is available for research use only.

Cell surface & cytoplasmic

Protein G affinity chromatography