NSJ Bioreagents

CDK4 Antibody

Product Code:
 
NSJ-F54049
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse lgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3D12-A7-F11
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store the CDK4 antibody at -20oC.
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Western blot testing of human 1) HeLa and 2) K562 cell lysates using CDK4 antibody at 1:500. Predicted molecular weight ~34 kDa.
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Western blot testing of human 1) HeLa and 2) K562 cell lysates using CDK4 antibody at 1:500. Predicted molecular weight ~34 kDa.

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NSJ-F54049-0.1ML0.1 ml£502.00
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Further Information

Application Details:
Western blot: 1:500
Application Note:
The stated application concentrations are suggested starting points. Titration of the CDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. [UniProt]
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant protein was used as the immunogen for this CDK4 antibody.
Limitation:
This CDK4 antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P11802

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